论文总字数：24622字

摘 要

目的：癌症是目前威胁人类生命健康最可怕的疾病之一，由于癌症晚期治疗效果差，治愈率极低，癌症早期的发现和治疗，成为延长患者生存期和治愈的关键。近年来荧光成像因为其优良的成像能力，成为临床肿瘤诊断及生物成像研究中不可或缺的一部分。传统的有机和无机染料由于某些缺陷，在生物成像领域应用受到了限制，因此需要开发具有良好光稳定性和生物相容性的新型荧光染料。

方法：本实验中，我们设计并合成了一种新型有机染料TPACN，并将其用生物相容性良好的聚合物泊洛沙姆F127包裹，形成纳米染料T_nano。对T_nano的尺寸、形貌和光学性质进行详细的表征，并考察其稳定性、生物相容性和体外成像能力。

结果：与游离的TPACN相比，T_nano在450 nm激发下可在608 nm发射出强烈的荧光，摩尔消光系数4.1×10⁴ M⁻¹cm⁻¹，荧光量子产率为0.67，还显著提高了染料在水溶液中的稳定性，增强其生物相容性，并能用于体外成像。

结论：T_nano优异的光学性质，以及显著的光稳定性和良好的生物相容性，可用于体外成像，在未来可进一步探究其在临床诊断方面的应用。

关键词：肿瘤诊断 荧光成像 纳米造影剂 有机荧光染料 生物相容性

Construction and Imaging of a new Nanocontrast Fluorescent dye

Abstract

Objectives: Cancer is currently the one of the most terrible disease threatening human life and health. Due to the poor treatment effect in the late stage of cancer and the extremely low cure rate, the early detection and treatment of cancer has become the key to prolong the survival and cure of patients. In recent years, fluorescence imaging has become an indispensable part of clinical tumor diagnosis and biological imaging research because of its excellent imaging ability. The traditional organic and inorganic dyes are limited in the field of biological imaging due to some defects, so it is necessary to develop new fluorescent dyes with good photostability and biocompatibility.

Methods: In this experiment, we designed and synthesized a new type of organic dye TPACN, and wrapped it with Pluronic F127, a polymer with good biocompatibility, to form a nanocontrast agent T_nano. The size, morphology and optical properties of T_nano were characterized in detail, and its stability, biocompatibility and in vitro imaging capability were investigated.

Results: Compared with free TPACN, the maximum fluorescence emission of T_nano at 608 nm with 450 nm excitation, Molar extinction coefficient of T_nano was found to be 4.1×10⁴ M⁻¹cm⁻¹, and its fluorescence quantum yield (QY) was 0.67. It can also improve the stability of TPACN in aqueous media significantly, enhances its biocompatibility, and can be used for in vitro imaging.

Conclusions: Excellent optical properties of T_nano, as well as its remarkable optical stability and good biocompatibility, can be used for in vitro imaging, and its application in clinical diagnosis can be further explored in the future.

Key Words: Tumor diagnosis; Fluorescence imaging; Nanocontrast agent; Organic fluorescent dye; Biocompatibility

目 录

摘要 I

Abstract II

第一章 文献综述 1

1.1 研究背景 1

1.2 肿瘤诊断技术 1

1.3 荧光染料的简介及分类 2

1.3.1 无机荧光染料 3

1.3.2 有机荧光染料 3

1.4 纳米化有机荧光染料 4

1.4.1 有机荧光染料纳米化构筑方法 5

1.4.2 纳米化荧光染料的应用 6

1.5 本论文的研究内容与意义 6

第二章 实验方法 7

2.1 实验仪器与试剂 7

2.2 TPACN的合成 9

2.3 TPACN的纳米化构筑(T_nano) 9

2.4 T_nano的表征 10

2.5 T_nano荧光量子产率(QY)和摩尔消光系数(ε)的测定 10

2.6 T_nano的稳定性试验 10

2.7 T_nano的生物相容性试验 11

2.8 T_nano的体外成像研究 11

2.9 实验数据分析与统计 11

第三章 结果与讨论 12

3.1 TPACN的光学性质 12

3.2 T_nano的制备和表征 12

3.3 T_nano的稳定性 14

3.4 T_nano的生物相容性 17

3.5 T_nano的体外成像能力 18

第四章 结论与展望 20

参考文献 21

致谢 24

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