1. 毕业设计（论文）的内容和要求

1、 内容 在试验过程中，制定出一套试验方案，也就是具体步骤，即：1、动物给药造模2、动物模型验证3、机制研究4、数据处理。

本实验以雄性sd大鼠为研究对象，探讨奥硝唑对大鼠的生殖损伤，并研究其作用机制。

2、 要求 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier电子期刊，pub med，sci等检索资源上查阅关于雷公藤甲素毒性及小鼠睾丸损伤的相关文献。

2. 参考文献

[1] Bo Ma, Huanhuan Qi, Jing Li, Hong Xu, Bo Chi, Jianwei Zhu, Lisha Yu, Guohua An,Qi Zhang. Triptolide disrupts fatty acids and peroxisome proliferator-activated receptor (PPAR) levels in male mice testes followed by testicular injury: A GC-MS based metabolomics study. Toxicology. 2015. 336: 84-95. [2] Imamovic KS， Pinter B． Ｒeview of clinical trials on effects of oral antioxidants on basic semen and other parameters in idiopathic oligoasthenoteratozoospermia. Biomed Ｒes Int， 2014，2014: 426951． [3] Chohan KＲ， Griffin JT， Lafromboise M， et al． Comparison of chromatin assays for DNA fragmentation evaluation in human sperm. J Androl, 2006, 27 (1) : 53-59． [4] Jurewicz J， Ｒadwan M， Sobala W， et al． The relationship between exposure to air pollution and sperm disomy. Environ Mol Mutagen, 2015, 56( 1) : 50-59． [5] Cavallini G． Male idiopathic oligoasthenoteratozoospermia. Asian J Androl， 2006，8(2) : 143-157 [6] Li J, Jin J, Li M, Guan C, Wang W, Zhu S, Qiu Y, Huang M, Huang Z, Role of Nrf2 in protection against triptolide-induced toxicity in rat kidney cells. Toxicol Lett.213 (2012) 194-202. [7] 庞雪冰，李红钢，朱继望，等. 奥硝唑对大鼠精子的影响及作用机制探讨[J]. 中华男科学杂志, 2005, 11(1): 26-28. [8] Pan J, RNA polymerase - an important molecular target of triptolide in cancer cells. Cancer Lett.292 (2010)149-152. [9] Phillips BT, Gassei K, Orwig KE. Spermatogonial stem cell regulation and spermatogenesis[J]. Philos Trans Ｒ Soc Lond B Biol Sci, 2010, 365 ( 1546) :1663-1678. [10] Castro-Santos P, Laborde CM, D#237;az-Peha R. Genomics, proteomics and metabolomics: their emerging roles in the discovery and validation of rheumatoid arthritis biomarkers[J]. Clin Exp Rheumatol, 2015, 33 (2): 279-286. [11] Patti GJ, Yanes O, Siuzdak G. Innovation: Metabolomics: the apogee of the omics trilogy. Nat Rev Mol Cell Biol, 2012, 13( 4) : 263-269. [12] Aitken RJ, Whiting S, De Iuliis GN, et al. Electrophilic aldehydes generated by sperm metabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase. J Biol Chem, 2012, 287( 39) : 33048-33060.

3. 毕业设计（论文）进程安排

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