1. 毕业设计（论文）的内容和要求

1. 毕业论文的要求 苯并吡喃衍生物具有广泛的生物和药理活性，可用于抗菌，抗肿瘤，治疗艾滋病，阿尔兹海默症等。

目前，合成苯并吡喃衍生物的方法多种多样，比如酒石酸钾钠催化，有机碱催化，沸石催化，离子液体催化，纳米粒子催化，微波辐射等。

这些化学催化法通常使用有毒或易挥发的有机溶剂，催化剂制备困难且难以重复使用，需要较高的反应温度。

2. 参考文献

1. Ding Y, Huang H, Hu Y, et al. New progress on lipases catalyzed C-C bond formation reactions[J]. Chinese Journal of Organic Chemistry, 2013, 33 (5): 905-914. 2. Lopez-Iglesias M, Gotor-Fernandez V. Recent advances in biocatalytic promiscuity: hydrolase-catalyzed reactions for nonconventional transformations[J]. Chemical Record, 2015, 15 (4): 743-759. 3. Miao Y, Rahimi M, Geertsema E M, Poelarends G J, et al. Recent developments in enzyme promiscuity for carbon-carbon bond-forming reactions[J]. Current Opinion in Chemical Biology, 2015, 25, 115-123. 4. Abbaspour-Gilandeh E, Aghaei-Hashjin M, Yahyazadeh A, et al. (CTA)3[SiW12]#8211;Li #8211;MMT: a novel, efficient and simple nanocatalyst for facile and one-pot access to diverse and densely functionalized 2-amino-4H-chromene derivatives via an eco-friendly multicomponent reaction in water[J]. RSC Advances, 2016, 6 (60): 55444-55462. 5. Sedaghat M E, Rajabpour Booshehri M, Nazarifar M R, et al. Surfactant modified bentonite (CTMAB-bentonite) as a solid heterogeneous catalyst for the rapid synthesis of 3,4-dihydropyrano[c]chromene derivatives[J]. Applied Clay Science, 2014, 95, 55-59. 6. Nagalapalli R, Jaggavarapu S R, Jalli V P, et al. Ultrasound promoted green and facile one-pot multicomponent synthesis of 3,4-dihydropyrano[c]chromene derivatives[J]. Journal of Chemistry, 2013, 2013, 1-8. 7. Kanakaraju S, Prasanna B, Basavoju S, et al. Ammonium acetate catalyzed an efficient one-pot three component synthesis of pyrano[3,2-c]chromene derivatives[J]. Arabian Journal of Chemistry, 2017, 10, S2705-S2713. 8. Azizi K, Heydari A. A simple, green, one-pot synthesis of magnetic-nanoparticle-supported proline without any source of supplemental linkers and application as a highly efficient base catalyst[J]. RSC Advances, 2014, 4 (13): 6508-6512. 9. Khan T, Siddiqui Z N. Perchloric acid modified-cellulose: a versatile, novel and biodegradable heterogeneous solid acid catalyst for single-pot synthesis of novel bis-pyran annulated heterocyclic scaffolds under solvent-free conditions[J]. New Journal of Chemistry, 2014, 38 (10): 4847-4858. 10. Tabatabaeian K, Zanjanchi M A, Mamaghani M, et al. Anchoring of ruthenium onto imine-functionalized zeolite beta: an efficient route for the synthesis of 4H-benzo[b]pyrans and pyrano[c]chromenes[J]. Canadian Journal of Chemistry 2014, 92 (11): 1086-1091. 11. Mayank, Kaur Billin B, Agnihotri P K, et al. Ionic liquid-coated carbon nanotubes as efficient metal-free catalysts for the synthesis of chromene derivatives[J]. ACS Sustainable Chemistry Engineering, 2018, 6 (3): 3714-3722. 12. Dekamin M G, Eslami M, Maleki A. Potassium phthalimide-N-oxyl: a novel, efficient, and simple organocatalyst for the one-pot three-component synthesis of various 2-amino-4H-chromene derivatives in water[J]. Tetrahedron, 2013, 69 (3): 1074-1085. 13. Brahmachari G, Laskar S, Banerjee B. Eco-friendly, one-pot multicomponent synthesis of pyran annulated heterocyclic scaffolds at room temperature using ammonium or sodium formate as non-toxic catalyst[J]. Journal of Heterocyclic Chemistry, 2014, 51 (S1): E303-E308. 14. Esmaeilpour M, Javidi J, Dehghani F, et al. A green one-pot three-component synthesis of tetrahydrobenzo[b]pyran and 3,4-dihydropyrano[c]chromene derivatives using a Fe3O4@SiO2#8211;imid#8211;PMAn magnetic nanocatalyst under ultrasonic irradiation or reflux conditions[J]. RSC Advances, 2015, 5 (34): 26625-26633.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 2019.01-2019.02 完成文献检索和开题报告 2019.01-2019.03 建立HPLC分析方法，对实验室现有的几种脂肪酶进行筛选 2019.04-2019.05 对脂肪酶催化芳香醛、丙二腈和4-羟基香豆素的反应的水含量、酶浓度、反应温度、反应时间等工艺条件进行优化 2019.05-2019.06 对底物进行扩展，得到分离产物，质谱、氢谱、碳谱进行结构表征 2019.06 数据整理、论文写作和答辩