1. 毕业设计（论文）的内容和要求

论文内容

糖基转移酶广泛存在于生物中，是生物体内实现糖基化的主要酶类。本实验室构建的含糖基转移酶zs1的大肠杆菌工程菌，能够催化酪醇糖基化合成红景天苷。该反应的关键性限制因素在于底物udpg十分昂贵，而利用蔗糖合成酶ss构建辅酶循环体系，可以实现udpg的循环再生。计划通过双酶融合体的构建，实现zs1和ss双功能酶的表达，并对其酶学性质进行研究。

论文要求

2. 参考文献

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[2] son mh, kim bg, kim dh, et al. production of flavonoid o-glucoside using sucrose synthase and flavonoid o-glucosyltransferase fusion protein[j]. journal of microbiology and biotechnology, 2009,19(7): 709-712.

[3] adlakha n, sawant s, anil a, et al. specific fusion of beta-1,4-endoglucanase and beta-1,4-glucosidase enhances cellulolytic activity and helps in channeling of intermediates[j]. applied and environmental microbiology, 2012,78(20): 7447-7454.

3. 毕业设计（论文）进程安排