1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、springer link全文电子期刊等检索资源，查阅关于生物转化皂苷类经典天然产物方面的相关文献。

2．文献阅读及综述 阅读与课题相关的外文与中文文献，了解国内外的研究动态，并进行文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，参考制定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

1) 吴薛明，何冰芳. 耐有机溶剂极端微生物及酶类与天然产物的生物转化,中国天然药物，2007,5,245-250. 2) 杜晓光, 耿美玉. 糖类药物，生命科学，2011,7,671-677. 3) 郑 翠，李 琳，庞 浩，王兆梅，天然产物活性组分的糖基化修饰研究进展，纤维素科学与技术，2012，20:62-71. 4) 邓玲玲，李轶，林贝，付岩帅，肖敏，陈畅，糖基化药物与糖基化技术的研究进展，中国生化药物杂志，2011,32,72-75. 5) Jing-yuan Liu, , Li Lu, , Li-ping Kang, Yi-xun Liu, Yang Zhao, Cheng-qi Xiong, Yu-qin Zhang, Li-yan Yu, Bai-ping Ma, Selective glycosylation of steroidal saponins by Arthrobacter nitroguajacolicus. Carbohydrate Research, 402, 2015, 71#8211;76. 6) Jin-Kwang Kim, Chang-Hao Cui, Min-Ho Yoon, Sun-Chang Kim, Wan-Taek Im. Bioconversion of major ginsenosides Rg1 to minor ginsenoside F1 using novel recombinant ginsenoside hydrolyzing glycosidase cloned from Sanguibacter keddieii and enzyme characterization, Journal of Biotechnology 161, 2012, 294#8211;301. 7) Wen-zhi Yang, Ying Hu, Wan-ying Wu, Min Ye, De-an Guo. Saponins in the genus Panax L. (Araliaceae): A systematic review of their chemical diversity, Phytochemistry 106, 2014, 7#8211;24. 8) 杨金玲, 高丽丽, 朱平,人参皂苷生物合成研究进展, 药学学报 Acta Pharmaceutica Sinica 2013, 48 (2): 170#8722;178 9) 何道同，王兵，陈珺明. 人参皂苷药理作用研究进展. 辽宁中医药大学学报, 2012,7:118-121. 10) Se-Hwa Kim, Jin-Woo Min, Lin-Hu Quan, Sungyoung Lee, Dong-Uk Yang, and Deok-Chun Yang. Enzymatic Transformation of Ginsenoside Rb1 by Lactobacillus pentosusStrain 6105 from Kimchi, J Ginseng Res.2012, 3, 291-297. 11) Jin-Kwang Kim, Chang-Hao Cui, Min-Ho Yoon, Sun-Chang Kim, Wan-Taek Im. Bioconversion of major ginsenosides Rg1 to minor ginsenoside F1 using novel recombinant ginsenoside hydrolyzing glycosidase cloned from Sanguibacter keddieii and enzyme characterization. Journal of Biotechnology 161, 2012, 294-301. 12) Liang Wanga, Qing-Mei Liua, Bong-Hyun Sungb, Dong-Shan Anc, Hyung-Gwan Leea, Song-Gun Kimc, Sun-Chang Kima,d, Sung-Taik Leea, Wan-Taek Im, Bioconversion of ginsenosides Rb1, Rb2, Rc and Rd by novel glucosidase hydrolyzing outer 3-O glycoside from Sphingomonas sp. 2F2: Cloning, expression, and enzyme characterization. Journal of Biotechnology 156, 2011,125-133. 13) Hao Hong, Chang-Hao Cui, Jin-Kwang Kim, Feng-Xie Jin, Sun-Chang Kim, and Wan-Taek Im, Enzymatic Biotransformation of Ginsenoside Rb1 and Gypenoside XVII into Ginsenosides Rd and F2 by Recombinant β-glucosidase from Flavobacterium johnsoniae. J Ginseng Res. 2012, 4, 418-424 14) Myung Keun Park, Chang-Hao Cui, Sung Chul Park2, Seul-Ki Park3, Jin-Kwang Kim, Mi-Sun Jung, Suk-Chae Jung, Sun-Chang Kim and Wan-Taek Im, Characterization of Recombinant β-Glucosidase from Arthrobacter chlorophenolicus and Biotransformation of Ginsenosides Rb1, Rb2, Rc, and Rd. Journal of Microbiology,2014,52, 399-406. 15) Zhubo Dai, Beibei Wang, Yi Liu, Mingyu Shi, Dong Wang, Xianan Zhang, Tao Liu, Luqi Huang and Xueli Zhang. Producing aglycons of ginsenosides in bakers#8217; yeast. Scientific Reports 4, Article number: 3698 doi:10.1038/srep03698.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2015.12.29-期末 文献查阅及翻译，完成开题报告，熟悉实验仪器。

2016.3.1.-2016.3.12. 人参皂苷原料准备，总皂苷提取富集及含量测定 2016.3.13.-2016.3.29. 总皂苷水解反应，得到原人参苷元并测定纯度 2016.3.30.-2016.4.15. 利用耐有机溶剂糖苷酶非水相酶法合成人参皂苷，产物结构鉴定 2016.4.15.-2016.5.10. 酶法反应条件优化 2016.5.11-2016.5.20. 最适反应下放大反应研究，考察反应得率和转化率 2016.5.21-2016.5.24. 目标产物分离纯化 2016.5.25.-2016.6.7. 撰写毕业设计论文，准备答辩