短肽水凝胶-石墨烯复合载体的制备及表征任务书
2020-06-27 19:36:45
1. 毕业设计(论文)的内容和要求
水凝胶是以水为分散介质,具有三维网状结构的交联聚合物,性质柔软,能够吸收大量的水保持一定的形状。
近年寡肽水凝胶由于其具有无免疫性、生物可降解性、生物相容性等性质使得水凝胶在生物传感器、药物控释、组织工程、三维细胞培养等领域具有潜在的应用价值。
本论文通过酶促法合成寡肽水凝胶,并对水凝胶性质进行研究,最后将此凝胶用于体外细胞培养。
2. 参考文献
[1]张琦, 张耀东. 酶启动和调控自组装超分子水凝胶[J]. 生命的化学, 2011, 31(4):587-591. [2]Williams R J, Smith A M, Collins R, et al. Enzyme-assisted self-assembly under thermodynamic control[J]. Connective Tissue Research, 2009, 4(1):19-24. [3]Williams R J, Hall T E, Glattauer V, et al. The in vivo performance of an enzyme-assisted self-assembled peptide/protein hydrogel.[J]. Biomaterials, 2011, 32(22):5304-10. [4]Xiao G, Christopher B W, Lei T, et al. Preparation and characterization of a novel sodium alginate incorporated self-assembled Fmoc-FF composite hydrogel[J]. Materials Science amp; Engineering C Materials for Biological Applications, 2016, 58(9):478. [5]Mie M, Oomuro M, Kobatake E. Hydrogel scaffolds composed of genetically synthesized self-assembling peptides for three-dimensional cell culture[J]. Polymer Journal, 2013, 45(5):504-508. [6]Jayawarna V, Ali M, Jowitt T, et al. Nanostructured Hydrogels for Three‐Dimensional Cell Culture Through Self‐Assembly of Fluorenylmethoxycarbonyl#8211;Dipeptides[J]. Advanced Materials, 2006, 18(5):611-614. [7]Kraehenbuehl T P, Ferreira L P. Cell-responsive hydrogel for encapsulation of vascular cells[J]. Biomaterials, 2009, 30(26):4318-24. [8]Wang H, Yang Z. Short-peptide-based molecular hydrogels: novel gelation strategies and applications for tissue engineering and drug delivery.[J]. Nanoscale, 2012, 4(17):5259-67. [9] Hauser C A, Zhang S. Designer self-assembling peptide nanofiber biological materials[J]. Chemical Society Reviews, 2010, 39(8):2780-90. [10] Toledano S, Williams R J, Vineetha Jayawarna A, et al. Enzyme-Triggered Self-Assembly of Peptide Hydrogels via Reversed Hydrolysis[J]. Journal of the American Chemical Society, 2006, 128(4):1070-1. [11] Panda J J, Mishra A, Basu A, et al. Stimuli responsive self-assembled hydrogel of a low molecular weight free dipeptide with potential for tunable drug delivery.[J]. Biomacromolecules, 2008, 9(8):2244. [12] Wang J, Wang Z, Gao J, et al. Incorporation of supramolecular hydrogels into agarose hydrogels - A potential drug delivery carrier[J]. Journal of Materials Chemistry, 2009, 19(42):7892-7896. [13] 白靖琨, 盛成乐, 张宇,等. 酶响应型肽水凝胶及应用研究进展[J]. 生物化学与生物物理进展, 2016, 43(11):1048-1060. [14] Li-Shan W, Fan L, Jaehong L, et al. Enzymatic conjugation of a bioactive peptide into an injectable hyaluronic acid-tyramine hydrogel system to promote the formation of functional vasculature[J]. Acta Biomaterialia, 2014, 10(6):2539. [15] Li S, Chen N, Zhang Z, et al. Endonuclease-responsive aptamer-functionalized hydrogel coating for sequential catch and release of cancer cells[J]. Biomaterials, 2013, 34(2):460.
3. 毕业设计(论文)进程安排
2017.12~2018.1 查阅、收集文献资料 2018.2~2018.3 理论学习及总体方案规划 2018.3~2018.4 使用本实验室已有菌种及发酵方法获得酶液并催化合成水凝胶。
2018.4~2018.4 对此水凝胶的性质进行研究 2018.4~2018.5 体外细胞培养 2018.5~2018.5 论文撰写
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