1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier、ncbi电子期刊，springer link全文电子期刊，pubmed等检索资源上查阅关于遗传转化体系构建的相关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] I. Banu, A. Lupu, I. Aprodu, DEGRADATION OF ZEARALENONE BY LACCASE ENZYME, Scientific Study Research Chemistry Chemical Engineering Biotechnology Food Industry, 14 (2014) 79-84. [2] A. Brodehl, A. Mouml;ller, H.J. Kunte, M. Koch, R. Maul, Biotransformation of the mycotoxin zearalenone by fungi of the genera Rhizopus and Aspergillus, Fems Microbiology Letters, 359 (2015) 124-130. [3] K.J. Cho, J.S. Kang, W.T. Cho, C.H. Lee, J.K. Ha, K.B. Song, In vitro degradation of zearalenone by Bacillus subtilis, Biotechnology Letters, 32 (2010) 1921-1924. [4] H. Tan, Z. Zhang, Y. Hu, L. Wu, F. Liao, J. He, B. Luo, Y. He, Z. Zuo, Z. Ren, G. Peng, J. Deng, Isolation and characterization of Pseudomonas otitidis TH-N1 capable of degrading Zearalenone, Food Control, 47 (2015) 285-290. [5] S.E. Tinyiro, C. Wokadala, D. Xu, W. Yao, Adsorption and degradation of zearalenone by bacillus strains, Folia Microbiologica, 56 (2011) 321. [6] S.E. Tinyiro, W. Yao, X. Sun, C. Wokadala, S. Wang, Scavenging of Zearalenone by Bacillus Strains-in vitro, Research Journal of Microbiology, 6 (2011) 304-309. [7] L. Abrunhosa, A. Venacirc;ncio, Isolation and purification of an enzyme hydrolyzing ochratoxin A from Aspergillus niger, Biotechnology Letters, 29 (2007) 1909-1914. [8] H. Zhang, M. Dong, Q. Yang, M.T. Apaliya, J. Li, X. Zhang, Biodegradation of zearalenone by Saccharomyces cerevisiae: Possible involvement of ZEN responsive proteins of the yeast, Journal of Proteomics, 143 (2016) 416-423. [9] I. Vanhoutte, K. Audenaert, G.L. De, Biodegradation of Mycotoxins: Tales from Known and Unexplored Worlds, Frontiers in Microbiology, 7 (2016) 561. [10] 唐语谦, 钟凤, 陈艺, 史成超, 李丹, 吴晖, 玉米赤霉烯酮生物脱毒及关键酶作用机理的研究进展, 现代食品科技, (2013) 1742-1746.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2016.1.2-2016.1.19 文献查询和资料阅读 2016.1.19-2016.1.23 研究方案制定和开题报告写作 2016.1.24-2016.3.10 实践内容的准备 2016.3.11-2016.5.10 实践 2016.5.10-2016.5.15 实践内容处理与结果分析 2016.5.16-2016.6.1 毕业论文书写