论文总字数：16561字

摘 要

关键词：苍术；寡糖；AKTA；HPLC；

A series of oligosaccharides and structural characterization in atractylodes

Abstract：As a traditional Chinese medicine, Atractylodes lancea has a wide range of pharmacological effects and has a good application prospect in clinical practice. However, the chemical composition of Atractylodes is very complicated. At present, research topics for fat-soluble substances in Atractylodes macrocephala are in the majority, and there are few issues that aim at the water-soluble components contained in Atractylodes lancea. However, the clinical application of Atractylodes Rhizome is mostly decoction. Modern pharmacological results also confirm that Atractylodes aqueous extract has good The pharmacological activity. This experiment focused on the in-depth study of the oligosaccharide contained in the aqueous extract of Atractylodes. The oligosaccharides were extracted using the more common ethanol reflux method to optimize the extraction conditions and improve the extraction efficiency. At the same time, the oligosaccharides extracted from the protein purification system (AKTA) and the semi-preparative solution were separated and purified, and detected by high performance liquid chromatography (HPLC). Structural characterization of oligosaccharide extracts was then performed using acid hydrolysis coupled with liquid phase analysis and methylation analysis. The experimental results show that ethanol refluxing method can effectively extract oligosaccharides from Atractylodes Rhizome, and AKTA and semi-preparation liquids have good separation and purification effects relative to oligosaccharides. In addition, through the monosaccharide composition experiment, it was found that the atractylotic oligosaccharide was a chrysanthemum-type oligosaccharide, and the glycosidic bond type was identified by a methylation experiment.

Keywords: oligosaccharide; Atractylodislancea; AKTA; HPLC

目录

苍术中系列寡糖的分离纯化与结构表征 I

第一章 文献综述 3

1. 苍术寡糖概述 3

1.1 苍术寡糖简介 3

1.2.1国内外研究概况 3

1.2.2 结构表征 3

1.2.3 苍术中的系列寡糖的理化性质研究概况 4

1.3 不同实验条件对苍术中寡糖的提取以及分离纯化的影响 4

1.3.1 不同药液比对苍术中寡糖提取的影响 4

1.3.2 不同溶剂比例对苍术中寡糖提取的影响 5

1.3.3 不同反应时间对苍术中寡糖提取的影响 5

1.3.4 柱填料对苍术中的寡糖的分离和纯化的影响 5

1.3.5 流速对苍术中的寡糖的分离和纯化的影响 5

1.3.6 温度对苍术中的寡糖的分离和纯化的影响 5

1.4 本研究的内容和意义 5

1.4.1 研究内容 5

1.4.2 研究意义 6

第二章 实验材料 7

2.1 实验仪器 7

2.2 实验试剂 8

第三章 实验方法 10

3.1 苍术中寡糖的提取 10

3.1.1 苍术样品的制备 10

3.1.2 提取溶剂的配制 1

3.1.3 提取寡糖 1

3.1.4 寡糖提取条件的优化 10

3.1.5 薄层色谱法检测寡糖的DP值 11

3.1.6苯酚硫酸法检测总糖含量 12

3.2苍术中寡糖的分离纯化 1

3.2.1 分离样品 1

3.2.2 苯酚硫酸法检测分离效果 1

3.2.3 高效液相色谱法检测分离效果 1

3.3 苍术中寡糖的结构表征 1

3.3.1 单糖组成的测定 14

3.3.2 甲基化反应 1

3.3.3 GC-MS分析 1

第四章 结果与讨论 1

4.1 提取条件优化结果 1

4.2 薄层色谱法检测结果 20

4.3 苯酚硫酸法测定总糖含量 21

4.4 苯酚硫酸法检测AKTA分离结果 22

4.5 HPLC-ELSD法检测AKTA分离结果 1

4.6 结构表征 1

4.6.1 单糖组成测定结果 25

4.6.2 GC-MS检测结果 27

第五章 结论与展望 1

5.1 结论 1

5.2 展望 1

参考文献 1

致谢 1

第一章文献综述

1.1苍术寡糖简介

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