1. 毕业设计（论文）的内容和要求

1、毕业论文内容 氯酚类污染物(chlorophenols，cps)是一类具有环境毒性的难降解有机物，这类化合物对人体、动植物、微生物等具有很强的毒性、致癌性及致突变性，即使在低浓度时也会对人体造成危害。

由于该类物质在水中的溶解度大，结构稳定，不易分解和转化，因此在自然界中不断累积，给自然环境造成了较大的危害。

目前常见的降解氯酚的方法有絮凝法、溶剂萃取法、吸附法、光化学氧化法、离子交换法以及超声波催化降解法等。

2. 参考文献

[1] Partha S. M. , Gupta S. K. Effect of influent p H and alkalinity on the removal of chlorophenols in sequential anaerobic-aerobic reactors[J]. Bioresource Technology, 2009, 100(5): 1881-1883. [2] Mansoor A. , Maryam L. Synthesis of amino-modified ordered mesoporous silica as a new nano sorbent for the removal of chlorophenols from aqueous media[J]. Chemical Engineering Journal, 2009,150(3): 555-560. [3] Hu J. , Wang L. J. Degradation of chlorophenols in aqueous solution by γ-radiation[J]. Radiation Physics and Chemistry, 2007, 76(9): 1489-1492. [4] Zhou T. ,Li Y. Z. , Wong F. S. , et al. Enhanced degradation of 2,4-dichlorophenol by ultrasound in a new Fen-ton like system (Fe / EDTA) at ambient circumstance[J]. Ultrasonics Sonochemistry, 2008, 15(5): 782-790. [5] Lu Q. F. , Yu J. , Gao J. Z. Degradation of 2, 4-dichlorophenol by using glow discharge electrolysis[J]. Journal of Hazardous Materials, 2006, 136(3): 526-531. [6] Muriel A. , Youssef F. M. , Craig D. Removal estrogenic activity of natural and synthetic hormones from a municipal wastewater: Efficiency of horseradish peroxidases and laccase from Trametes versicolor[J]. Chemosphere, 2008, 70(3): 445-452. [7] Panneer S. S. , Anil K. , Rita K. An insilco approach to bioremediation: Laccase as a case study[J]. Journal of Molecular Graphics and Modelling, 2008, 26(5): 845-849. [8] Shigeyuki T. , Chiak S. , Kazuko S. Exoenzymes of Trametes versicolor can metabolize coplanar PCB congeners and hydroxy PCB[J]. Chemosphere, 2007, 67(9): 54-57. [9] Vianello F. , Ragusa S. , Cambria M. T. , et al. A high sensitivity amperometric biosensor using laccase as biorecognition element[J]. Biosensors and Bioelectronics, 2006, 21(11): 2155-2160. [10] Khani Z. , Jolivalt C. , Cretin M. , et al. Alginate/carbon composite beads for laccase and glucose oxidase encapsulation: Application in biofuel cell technology[J]. Biotechnology Letters, 2006, 28(22): 1779-1786. [11] Rogalski J. , Dawidowicz A. , Jozwik E. A. , et al, Immobilization of laccase from cerrena unicolor on controlled porosity glass[J]. Journal of Molecular Catalysis B: Enzymatic, 1999, 6(1-2): 29-39. [12] Zhang J. B. , Xua Z. Q. , Chen H. , et al, Removal of 2,4-dichlorophenol by chitosan-immobilized laccase from Coriolus versicolor[J]. Biochemical Engineering Journal, 2009, 45(1): 54-59. [13] 刘晓贞, 李 云, 梁云霄, 等. 二氧化硅/壳聚糖大孔复合材料固定化漆酶及其对2,4-二氯苯酚的降解[J]. 高校化学工程学报, 2016, 30(1): 201-209.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容2019.1-2019.2 完成文献检索和开题报告 2019.3-2019.4 建立酶活测定方法，制备载体并进行表征 2019.5-2019.6 漆酶固定化，固定化酶的酶学性能考察，固定化酶降解2,4-DCP考察 2019.6 数据整理、论文写作和答辩