[1] Review: Recombinant protein folding and misfolding in Escherichia coli

[2] The novel Fh8 and H fusion partner for soluble protein expression in E.coli : acomparison with the traditional gene fusion technology

[3]fusion tags for protin solubility,purification,and immunogenicity in Escherichia col: the novel Fh8 system

[4] unrelated solubility-enhancing fusion partners MBP and NusA Utilize a similar mode of action

[5] a support vector machine-based method for predicting the propensity of a protein to be soluble or to form inclusion body on overexpression in Escherichia coli.

[6] Characteristics of an Organic Solvent-Tolerant β#8209;Fructofuranosidase from Arthrobacter arilaitensis NJEM01 and Efficient Synthesis of Prebiotic Kestose

[7] Efficient enzymatic synthesis of mangiferin glycosides in hydrophilic organic solvents

[8] Xueming Wu, Jianlin Chu , Bin Wu, Sen Zhang, Bingfang He* (2012)An efficient novel glycosylation of flavonoid by b-fructosidase resistant to hydrophilic organic solvents, Bioresource Technology 129 (2013) 659#8211;662

[9] Xueming Wua,b,#8727;, Jianlin Chub, Tingting Xua, Bingfang He*(2013)Isolation, identification and pharmacokinetic analysis of fructosyl puerarins from enzymatic glycosylation

[10] Jianlin Chu, Xueming Wu, Bin Wu,Rui Wang, Bingfang He* (2014)Characteristics of an Organic Solvent-Tolerant β#8209;Fructofuranosidase from Arthrobacter arilaitensis NJEM01 and Efficient Synthesis of Prebiotic Kestose; J. Agric. Food Chem. 2014, 62, 5408#8722;5411

[11] Xueming Wu, Jianlin Chu, Bin Wu, Sen Zhang, Bingfang He* (2013)Efficient enzymatic synthesis of mangiferin glycosides in hydrophilic organic solvents, RSCAdv, DOI: 10.1039/c3ra42648c

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[13] Smialowski P, Doose G, Torkler P, Kaufmann S, Frishman D: PROSO II-a new method for protein solubility prediction. FEBS J 2012, 279(12):2192#8211;2200.

[14] Xiaohui N, Feng S, Xuehai H, Jingbo X, Nana L: Predicting the protein solubility by integrating chaos games representation and entropy in information theory. Expert Syst Appl 2014, 41(4):1672#8211;1679.

[15] Huang H, Charoenkwan P, Kao T, Lee H, Chang F, Huang W, Ho S, Shu L,Chen W, Ho S: Prediction and analysis of protein solubility using a novel scoring card method with dipeptide composition. BMC Bioinfomratics 2012, 13(17):S3.

起讫日期

设计（论文）各阶段工作内容

备 注

2015.12-2016.2

文献查阅，完成开题报告，熟悉实验仪器。

2016.3-2016.4

社区获得性呼吸窘迫综合症毒素的克隆

2016.4-2016.5

融合标签融合社区获得性呼吸窘迫综合症毒素表达载体的构建

2016.5-2016.6

表达条件的优化