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毕业论文网 > 任务书 > 化学化工与生命科学类 > 应用化学 > 正文

基于功能化石墨烯的酶传感器构建研究任务书

 2020-05-26 20:27:54  

1. 毕业设计(论文)的内容和要求

石墨烯是由碳原子堆积形成的平面晶体,其厚度仅是一个原子尺寸。石墨烯特殊的结构(单原子厚度、 sp2 杂化的原子轨道、离域的大 π 键)决定了其在 光学、电学、热学、力学等方面拥有许多优异的性能。石墨烯结构中含有大量的 π 键,层与层之间容易因为 π-π 堆积作用而相互堆叠在一 起,宏观上表现为石墨烯的团聚。较差的分散性限制了石墨烯的进一步应用,对石墨烯进行功能化,不仅可以解决石墨烯应用中遇到的这一问题,而且还可以改善石墨烯的部分性能(如负载能力、生物兼容性等)。

本课题设计通过对氧化石墨烯化学还原的同时引入聚赖氨酸(pll),合成稳定分散的氨基化石墨烯。同时,基于金纳米粒子高催化活性和优异的导电性,本课题设计通过hbtu/三乙胺交联剂将金纳米粒子固定到氨基化石墨烯表面,合成具有优异导电性和良好生物兼容性的纳米金@石墨烯复合材料。该复合材料将为酶的固定提供良好基底,为固定化酶良好的生物微环境,实现其自然构象的保持,促进酶活性中心与电极之间有效的电子传递过程。以此为基础构建具有良好检测性能的酶电化学生物传感器。

本课题选题属于分析、应用类型,难度适中,工作量较大。

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2. 参考文献

[1] Ma J, Wang X, Liu Y, et al. Reduction of graphene oxide with l-lysine to prepare reduced graphene oxide stabilized with polysaccharide polyelectrolyte [J]. J Mater Chem A, 2013, 1(6):2192.
[2] Liu H, Kuila T, Kim N H, et al. In situ synthesis of the reduced graphene oxide?polyethyleneimine composite and its gas barrier properties [J]. J Mater Chem A, 2013, 1(11):3739.
[3] Fernandez-Merino M J, Guardia L, Paredes J I, et al. Vitamin C is an ideal substitute for hydrazine in the reduction of graphene oxide suspensions [J]. J Phys Chem C, 2010, 114(14):6426-6432.
[4] M.D. Maksimovic, V.B. Miskovic-Stankovic, The corrosion behaviour of epoxy resin electrocoated steel, Corros. Sci. 33 (2) (1992) 271?79.
[5] S. Hiromoto, Self-healing property of hydroxyapatite and octacalcium phosphate coatings on pure magnesium and magnesium alloy, Corros. Sci.100 (2015) 284?94.
[6] L. Ma, F. Chen, Z. Li, M. Gan, J. Yan, S. Wei, Y. Bai, J. Zeng, Preparation and anticorrosion property of poly(2,3-dimethylaniline) modified by nano-SiO2, Compos. Part B: Eng. 58 (2014) 54?8.
[7] J. Wang, R. Xu, Y. Zhang, Influence of SiO2 nano-particles on microstructures and properties of Ni朩朠/CeO2朣iO2 composites prepared by pulse electrodeposition, Trans. Nonferrous Met. Soc. China 20 (5) (2010) 839?43.
[8] S. Sharifi Golru, M.M. Attar, B. Ramezanzadeh, Studying the influence of nano-Al2O3 particles on the corrosion performance and hydrolytic degradation resistance of an epoxy/polyamide coating on AA-1050, Prog. Org. Coat. 77 (9) (2014) 1391?399.
[9] L. Liu, J. Xu, A study of the erosion-corrosion behavior of nano-Cr2O3 particles reinforced Ni-based composite alloying layer in aqueous slurry environment, Vacuum 85 (6) (2011) 687?00.
[10] Dongmei Cui, Li Mi, Xuan Xu, Jusheng Lu, Jing Qian, and Songqin Liu. Nanocomposites of graphene and cytochrome P450 2D6 isozyme for electrochemical-driven tramadol metabolism. Langmuir 2014, 30, 11833-11840.
[11] Xuan Xu, Jiachao Yu, Jin Qian, Dongmei Cui, and Songqin Liu. Functionalization of nitrogen-doped carbon nanotubes by 1-pyrenebutyric acid and its application for biosensing. IEEE Sens J. 2014, 14(7), 2341-2346
[12] Henan Li, Yawen Mu, Junrong Yan, Dongmei Cui, Weijun Ou, Yakun Wan, and Songqin Liu. A label-free photoelectrochemical immunosensor for neutrophil gelatinase-associated lipocalin based on the novel use of nanobodies. Anal. Chem. 2015, 87, 2007-2015
[13] Jing Qian, Wei Zhu, Li Mi, Xuan Xu, Jiachao Yu, Dongmei Cui, Yanchun Xue, Songqin Liu. Nanohybrids of quantum dots and cytochrome P450 for light-driven drug metabolism. J. Electroanal. Chem. 2014, 733, 27-32.
[14] L.B. Zhang, J.Q. Wang, H.G. Wang, Y. Xu, Z.F. Wang, Z.P. Li, Y.J. Mi, S.R. Yang, Preparation, mechanical and thermal properties of functionalized graphene/polyimide nanocomposites, Composites A 43 (2012) 1537?545.
[15] I.H. Tseng, Y.F. Liao, J.C. Chiang, M.H. Chiang, Transparent polyimide/graphene oxide nanocomposite with improved moisture barrier property, Mater. Chem. Phys. 136 (2012) 247?53.
[16] L.B. Zhang, J.Q. Zhang, H.G. Wang, Y. Xu, Z.F. Wang, Z.P. Li, Y.J. Mi, S.R. Yang, Preparation, mechanical and thermal properties of functionalized graphene/polyimide nanocomposites, Compos. A ?Appl. Sci. Manuf. 43 (2012) 1537?545.
[17] L. Yan, Y.B. Zheng, F. Zhao, S. Li, X. Gao, B. Xu, P.S. Weiss, Y. Zhao, Chemistry and physics of a single atomic layer: strategies and challenges for functionalization of graphene and graphene-based materials, Chem. Soc. Rev. 41 (2012) 97?14.
[18] Y. He, C. Tong, L. Geng, L. Liu, C. L? Enhanced performance of the sulfonated polyimide proton exchange membranes by graphene oxide: size effect of graphene oxide, J. Membr. Sci. 458 (2014) 36?6.
[19] L.B. Zhang, J.Q. Wang, H.G. Wang, Y. Xu, Z.F. Wang, Z.P. Li, Y.J. Mi, S.R.Yang, Preparation, mechanical and thermal properties of functionalized graphene/polyimide nanocomposites, Composites A 43 (2012) 1537?545.
[20] N.D. Luong, U. Hippi, J.T. Korhonen, A.J. Soininen, J. Ruokolainen, L.S. Johansson, J.D. Nam, L.H. Sinh, J. Sepp鋖? Enhanced mechanical and electrical properties of polyimide film by graphene sheets via in situ polymerization, Polymer 52 (2011) 5237?242.

3. 毕业设计(论文)进程安排

2015.12.26-2016.3.1 查阅资料,完成开题报告和任务书
2016.3.2-2016.4.1 确定方案,学习实验过程和操作
2016.4.2-2016.5.13 独立实验,合成纳米材料并测试性能
2016.5.14-2016.6.12 数据整理,书写论文,制作PPT
2016.6.13- 修改论文并完成答辩

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