1. 毕业设计（论文）的内容和要求

硝酰基(nitroxyl, hno)作为活性氮簇分子的一员，能够脱去电子或者还原为一种众所周知的生物信号分子一氧化氮(no), 但到目前为止仍然缺少有效的检测手段来阐释其在活体生物中的存在和运载机理。

no作为衍生内皮舒张因子影响心脏调节功能已广为报导，而由德州大学医学院murad、纽约州立大学furchgott和加州大学洛杉矶分校ignarro主导发现no在心血管系统中的重要作用获得1998年度诺贝尔生理及医学奖。

近年来，其衍生物hno对心脏功能的影响才被发现。

2. 参考文献

[1] Rhee S G. Cell signaling: H2O2, a necessary evil for cell signaling [J]. Science, 2006, 312 (5782):1882-1883. [2] Fukuto J M, Dutton A S, Houk K N. The chemistry and biology of nitroxyl (HNO): a chemically unique species with novel and important biological activity [J]. ChemBioChem, 2005, 6(4): 612-619. [3] Reisz J A, Zink C N, King S B. Rapid and selective nitroxyl (HNO) trapping by phosphines: kinetics and new aqueous ligations for HNO detection and quantitation [J]. J. Am. Chem. Soc., 2011,133(30): 11675-11695. [4] Feelisch M. Nitroxyl gets to the heart of the matter[J]. Proc. Natl. Acad. Sci. U S A, 2003, 100(9): 4978-4980. [5] Espey M G, Miranda K M, Thomas D D, et al. Ingress and reactive chemistry of nitroxyl-derived species within human cells [J]. Free. Radic. Biol. Med., 2002, 33(6):827-834. [6] Shafirovich V, Lymar S V. Nitroxyl and its anion in aqueous solutions: spin states, protic equilibria, and reactivities toward oxygen and nitric oxide [J]. Proc. Natl. Acad. Sci. U S A, 2002,99(11):7340-7345. [7] Hughes M N, Cammack R. Synthesis, chemistry, and applications of nitroxyl ion releasers sodium trioxodinitrate or Angeli#8217;s salt and Piloty#8217;s acid [J]. Methods Enzymol., 1999,301:279-287. [8] Rosenthal J, Lippard S J. Direct detection of nitroxyl in aqueous solution using a tripodal copper(II) BODIPY complex [J]. J. Am. Chem. Soc., 2010,132 (16):5536-5537. [9] Zhou Y, Liu K, Li J Y, et al. Visualization of nitroxyl in living cells by a chelated copper(II) coumarin complex [J]. Org. Lett., 2011, 13(6):1290-1293. [10] Trenor S R, Shultz A R, Love B J, et al. Coumarins in polymers: from light harvesting to photo-cross-linkable tissue scaffolds [J]. Chem. Rev., 2004, 104(6): 3059-3077. [11] Pluth M D, Tomat E, Lippard S J. Biochemistry of mobile zinc and nitric oxide revealed by fluorescent sensors. Annual Review of Biochemistry. 2011, 80: 333-355. [12] King S B, Nagasawa H T. Chemical approaches toward generation of nitroxyl in: L. Packer (Ed.), Methods in Enzymology, Part C, vol. 301, Academic Press,San Diego, 1999, pp. 211-220. [13] Hughes M N, Nicklin H G. The chemistry of pernitrites. Part I. Kinetics of decomposition of pernitrous acid [J]. J. Chem. Soc. (A), 1968, 2: 450-452. [14] Fenton, H H. On a new reaction of tartaric acid [J]. Chem. News., 1876, 33, 190. [15] Benesi H A, Hildebrand J H. A spectrophotometric investigation of the interaction of iodine with aromatic hydrocarbons [J]. J. Am. Chem. Soc., 1949, 71(8): 2703-2707. [16] Yang Y K, Ko S K, Shin I, et al. Synthesis of a highly metal-selective rhodaminebased probe and its use for the in vivo monitoring of mercury [J]. Nature Protocols. 2007, 2(7): 1740-1745. [17] Taki M, Iyoshi S, Ojida A, et al. Development of highly sensitive fluorescent probes for detection of intracellular copper(I) in living systems [J]. J. Am. Chem. Soc., 2010, 132(17): 5938-5939. [18] Chen X, Ko S K, Kim M J, et al. A thiol-specific fluorescent probe and its application for bioimaging [J]. Chem. Commun., 2010, 46(16): 2751-2753.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2017.1.2-2017.3.1 查阅资料，完成开题报告和任务书 2017.3.2-2017.3.10 确定方案，学习实验过程和操作 2017.3.11-2017.4.30 独立实验，合成产品并进行光谱表征 2017.5.1-2016.7.30 数据整理，书写论文，制作PPT 2017.5.30- 修改论文并完成答辩