1. 毕业设计（论文）的内容和要求

一、论文内容： 甲壳素(chitin)又称几丁质。

为n-乙酰葡糖胺通过β-1，4糖苷键连接聚合而成的一种含氮多糖类生物性高分子。

主要的来源为虾、蟹、昆虫等甲壳类动物的外壳与软体动物的器官（例如乌贼的软骨），以及真菌类的细胞壁等。

2. 参考文献

[1] Brzeski MM. Chitin and chitosan-putting waste to good use. Infofish Int Mag1987; 5:31#8211;3. [2] Ornum JV. Shrimp waste-must it be wasted. Infofish Int Mag 1992; 6:48#8211;52. [3] 刘海燕，丁存宝，贾长虹，等.微生物几丁质酶的应用研究进展［J］.河北农业科学， 2008,12﹙11﹚:68#8722;69. [4] Healy MG, Romo CR, Bustos R. Bioconversion of marine crustacean shell waste. Resour Conserv Recy 1994; 11:139#8211;47. [5] Wang S, Moync A, Thottappilly G, Wu S, Locy RD, Singh NK. Purification and characterization of a Bacillus cereus exochitinase. Enzyme Microb Technol 2001; 28:492#8211;8. [6] Aam, B.B., Heggset, E.B., Norberg, A.L., Soslash;rlie, M., Varing;rum, K.M., Eijsink, V.G., 2010.Production of chitooligosaccharides and their potential applications inmedicine. Mar. Drugs 8, 1482#8211;1517. [7] 郭玉莲.微生物几丁质酶及其在植物病害防治中的作用.中国农学学通报,2005, 21（1）:283～285. [8] Chen, L., Zhou, Y., Qu, M., Zhao, Y., Yang, Q., 2014. Fully deacetylated chitooligosaccharides act as efficient glycoside hydrolase family 18 chitinase inhibitors. J. Biol. Chem. 289, 17932#8211;17940. [9] 陈亚成,陈红歌,王石磊.产碱性几丁质酶菌种的筛选与发酵条件的研究.河南农业大学学报,1998,32(4):389～393. [10] Liu, T., Liu, Z., Song, C., Hu, Y., Han, Z., She, J., Fan, F., Wang, J., Jin, C., Chang, J., Zhou, J.M., Chai, J., 2012. Chitin-induced dimerization activates a plant immune receptor. Science 336, 1160#8211;1164. [11] Ulhoa CJ, Peberdy JF. Purification and some properties of the extracellular chitinase produced by Trichoderma harzianum. Enzyme Microb Technol 1992; 14:236#8211;40. [12] 潘志强. 海洋产几丁质酶菌株的筛选、鉴定与发酵工艺的优化.华南热带农业大学硕士学位论文,2005.6. [13] Kadokura, K., Rokutani, A., Yamamoto, M., Ikegami, T., Sugita, H., Itoi, S., 2007. Purification and characterization of Vibrio parahaemolyticus extracellular chitinase and chitin oligosaccharide deacetylase involved in the production of hetero disaccharide from chitin. Microbiol. Biotechnol. 75, 357#8211;365. [14] Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 1959; 31:426#8211;8. [15] Katouno, F., Taguchi, M., Sakurai, K., Uchiyama, T., Nikaidou, N., Nonaka, T.,Sugiyama, J, Watanabe, T, 2004. Importance of exposed aromatic residues in chitinase B from Serratia marcescens 2170 for crystalline chitin hydrolysis. J. Biochem. 136, 163#8211;168.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 2016.12.19-2017.01.03 选题，与导师讨论题目，导师下达任务书 2017.01.06-2017.01.13 根据任务书要求，查找相关文献资料，完成开题报告 2017.01.13-05.22 完成试验并提交论文第一稿 2017.05.22-05.28 根据导师意见修改论文第一稿，完成并提交第二稿 2017.05.29-06.2 根据导师意见修改论文第二稿，完成并提交第三稿 2017.06.2-06.14 完成论文的审阅，准备答辩