1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier、ncbi电子期刊，springer link全文电子期刊，pubmed等检索资源上查阅关于遗传转化体系构建的相关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

1. Schoene C, Bennett S P, Howarth M. SpyRing interrogation: analyzing how enzyme resilience can be achieved with phytase and distinct cyclization chemistries.[J]. Scientific Reports, 2016, 6:21151. 2. Brune K D, Leneghan D B, Brian I J, et al. Plug-and-Display: decoration of Virus-Like Particles via isopeptide bonds for modular immunization[J]. Scientific Reports, 2016, 6. 3. Li, L., et al. (2014). "Structural analysis and optimization of the covalent association between SpyCatcher and a peptide Tag." J Mol Biol 426(2): 309-317. 4. Zhang, W. B., et al. (2013). "Controlling macromolecular topology with genetically encoded SpyTag-SpyCatcher chemistry." J Am Chem Soc 135(37): 13988-13997. 5. Botyanszki, Z., et al. (2015). "Engineered catalytic biofilms: Site-specific enzyme immobilization onto E. coli curli nanofibers." Biotechnol Bioeng. 6. Bijan Zakeria, J. O. F. (2011). "Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin." PNAS. 7. Liu, Z., et al. (2014). "A novel method for synthetic vaccine construction based on protein assembly." Sci Rep 4: 7266. 8. Li H, Zhu D, Hyatt B A. Cloning, Protein Sequence Clarification, and Substrate Specificity of a Leucine Dehydrogenase from Bacillus sphaericus ATCC4525[J]. Applied Biochemistry and Biotechnology, 2009, 158(2):343-51. 9. Li J, Pan J, Zhang J, et al. Stereoselective synthesis of l - tert -leucine by a newly cloned leucine dehydrogenase from Exiguobacterium sibiricum[J]. Journal of Molecular Catalysis B Enzymatic, 2014, 105(7):11-17. 10. Ohshima T, Nishida N, Bakthavatsalam S, et al. The purification, characterization, cloning and sequencing of the gene for a halostable and thermostable leucine dehydrogenase from Thermoactinomyces intermedius.[J]. European Journal of Biochemistry, 1994, 222(2):305-12.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2017.1.2-2017.1.19 文献查询和资料阅读 2017.1.19-2017.1.23 研究方案制定和开题报告写作 2017.1.24-2017.3.10 实践内容的准备 2017.3.11-2017.5.10 实践 2017.5.10-2017.5.15 实践内容处理与结果分析 2017.5.16-2017.6.1 毕业论文书写