1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、springer link全文电子期刊等检索资源，查阅关于木聚糖生产丁醇方面的相关文献。

2．文献阅读及综述 阅读与课题相关的外文与中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] Lee S.Y., Park J.H., Jang S.H., et al. Fermentative butanol production by Clostridia [J]. Biotechnol Bioeng, 2008, 101(2): 209#8722;228. [2] D#252;rre P. Biobutanol: an attractive biofuel [J]. Biotechnol J,2007,2:1525#8211;1534 [3] 刘 娅， 刘宏娟， 张建安等． 新型生物燃料--丁醇的研究进展[J]. 现代化工, 2008, 28(6): 28-33. [4] Chiao J.S., Sun Z.H. History of the acetone-butanol-ethanol fermentation industry in China: development of continuous production technology[J]. J Mol Microbiol Biotechnol, 2007, 13(1/3): 12#8722;14. [5] Ni Y., Sun Z.H. Recent progress on industrial fermentative production of acetone-butanol-ethanol by Clostridium acetobutylicum in China [J]. Appl Microbiol Biotechnol, 2009, 83(3): 415#8722;423. [6] Kirschner M., n-Butanol, Chemical Market Reporter, 2006, 30 Jan#8211;5 Feb, ABI/INFORM Global, p 42 [7] http://syjhs.askci.com/2011-07/18/16334237244644.shtml. 丁辛醇项目商业计划 书,中国商报网. [8] http://free.chinabaogao.com/huagong/201012/1211b0Z2010.html. 丁/辛醇国际及 国内市场运行情况分析, 中国报告网. [9] Donaldson G.K., Huang L.L., Maggio-Hall L.A., et al. Fermentative production of four carbon alcohols,2007, International Patent WO2007/041269. [10] Jones D.T., Woods D.R., Acetone-butanol fermentation revisited [J]. Microbiol Rev,1986, 50:484#8211;524. [11] 宋锦玉．新一代的生物燃料--丁醇的开发动向[J]. 当代化工， 2011, 40 (6): 631#8722;632. [12] Environmental Protection Agency. Regulation of Fuels and Fuel Additives: Changes to Renewable Fuel Standard Program; Final Rule [EB/OL]. http://www.betalabservices.com/PDF/US-EPA-RFS2.pdf. [13] 陈陶声， 陆祖棋． 发酵法丙酮和丁醇生产技术[M]. 北京: 化学工业出版社,1991 [14] Johnson J.L., Toth J., Santiwatanakul S., et al. Cultures of”Clostridium acetobutylicum” from various collections comprise Clostridium acetobutylicum, Clostridium beijerinckii, and two other distinct types based on DNA-DNA reassociation[J]. Int J Syst Bacteriol, 1997, 47(2): 420#8722;424. [15] Shaheen R., Shirley M., Jones D.T. Comparative fermentation studies of industrial strains belonging to four species of solvent-producing clostridia [J]. J Mol Microbiol Biotechnol, 2000, 2(1): 115#8722;124. [16] Gheshlaghi R., Scharer J.M., Moo-Young M., et al. Metabolic pathways of clostridia for producing butanol[J]. Biotechnol. Adv.2009, 27: 764-781. [17] Shinto H., Tashiro Y., Kobayashi G., et al. Kinetic study of substrate dependency for higher butanol production in acetone#8211;butanol#8211;ethanol fermentation[J]. Process Biochem. 2008, 43: 1452-1461. [18] Rao G., Mutharasan R. Altered Electron Flow in Continuous Cultures of Clostridium acetobutylicum Induced by Viologen Dyes[J]. Appl Environ Microbiol, 1987, 53(6): 1232-1235. [19] Tashiro Y., Sonomoto K.. Advances in butanol production by clostridia. Yukihiro Tashiro Kenji Sonomoto. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology (Microbiology Book Series, Volume 2), Antonio Mendez Vilas (ed.), ISBN (13): 978-84-614-6195-0, Formatex Research Center (Badajoz, Spain), p. 1383-1394 (2010.12). [20] Gheshlaghi R., Scharer J.M., Moo-Young M., et al. Metabolic pathways of clostridia for producing butanol. Biotechnol. Adv. 2009, 27: 764-781. [21] Garc#237;a V., Pakkila J., et al. Challenges in biobutanol production: How to improve the efficiency? [J]. Renew Sust energ rev, 2011,15(2): 964#8211;980.

3. 毕业设计（论文）进程安排

2017.12-2018.02，文献查阅，完成开题报告，熟悉实验仪器 2018.03-2018.04，筛选能降解木聚糖的菌群 2018.04-2018.05，不同碳源对菌群产丁醇的影响 2018.05-2018.06，丁醇产量的优化，并完成论文写作。