1. 毕业设计（论文）的内容和要求

1．相关文献的查阅、阅读 掌握文献查阅方法，正确使用网络资源如中国期刊网、维普数据库、elsevier电子期刊、springer link全文电子期刊等检索资源，查阅关于解脂耶氏酵母菌株的相关文献。

并阅读与本课题相关的期刊等，了解菌种特性、生长情况、国内外研究情况。

2．制定实验方案 根据所阅读的文献，充分了解课题菌株特性，确定实验内容和实验可行性方法，制定工作计划。

2. 参考文献

[1] NicaudJ M.Yarowia lipolytica[J].Yeast,2012,29:409#8212; 418. [2] Blazeck, J. et al. (2014) Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production. Nat. Commun. 5, 3131 [3] Abghari, A. and Chen, S. (2014) Yarrowia lipolytica as an oleagi- nous cell factory platform for the production of fatty acid-based biofuel and bioproducts. Front. Energ. Res. http://dx.doi.org/10. 3389/fenrg. 2014.00021 [4] Workman, M. et al. (2013) Comparing cellular performance of Yarrowia lipolytica during growth on glucose and glycerol in sub- merged cultivations. AMB Express 3, 58 [5] Lazar, Z. et al. (2011) Simultaneous production of citric acid and invertase by Yarrowia lipolytica SUC transformants. Bioresour. Technol. 102, 6982#8211;6989 [6] Lazar, Z. et al. (2014) Hexokinase #8211; a limiting factor in lipid production from fructose in Yarrowia lipolytica. Metab. Eng. 26C, 89#8211;99 [7] Mlickova, K. et al. (2004) Lipid accumulation, lipid body formation, and acyl coenzyme A oxidases of the yeast Yarrowia lipolytica. Appl. Environ. Microbiol. 70, 3918#8211;3924 [8] Ryu, S. et al. (2015) Activating and elucidating complex sugar metabolism in Yarrowia lipolytica. Appl. Environ. Microbiol. 82, 1334#8211;1345 [9] Kurtzman, C.P. (2011) Yarrowia van der Walt von Arx (1980). In The Yeasts (5th edn) (Boekhout, C.P.K.W.F., ed.), pp. 927#8211;929, Elsevier [10] Ledesma-Amaro, R. et al. (2015) Engineering Yarrowia lipolytica to produce biodiesel from raw starch. Biotechnol. Biofuels 8, 148 [11] Lane, S. et al. (2015) Development and physiological characteri- zation of cellobiose-consuming Yarrowia lipolytica. Biotechnol. Bioeng. 112, 1012#8211;1022 [12] Guo, Z. et al. (2015) Development of cellobiose-degrading ability in Yarrowia lipolytica strain by overexpression of endogenous genes. Biotechnol. Biofuels 8, 109 [13] Young, E. et al. (2011) Functional survey for heterologous sugar transport proteins, using Saccharomyces cerevisiae as a host. Appl. Environ. Microbiol. 77, 3311#8211;3319 [14] Zhiyong Cui, Cuijuan Gao, Jiaojiao Li, Jin Hou, Carol Sze Ki Lin, Qingsheng Qi, Metabolic Engineering (2017), Engineering of unconventional yeast Yarrowia lipolytica for efficient succinic acid production from glycerol at low pH, 126-133 [15] Svetlana V. Kamzolova Natalia G. Vinokurova Olga N. Shemshura Nadiya E. Bekmakhanova Julia N. Lunina Vladimir A. Samoilenko Igor G. Morgunov，Appl Microbiol Biotechnol （2014）, The production of succinic acid by yeast Yarrowia lipolytica through a two-step process, 98:7959-7969 [16] Ruiling Gao, Zifu Li,Xiaoqin Zhou, Shikun Cheng and Lei Zheng, Gao et al.Biotechnol Biofuels (2017), Oleaginous yeast Yarrowia lipolytica culture with synthetic and food waste-derived volatile fatty acids for lipid production, 10:247 [17] Bligh E, Dyer WJ., A rapid method of total lipid extraction and purification. Can J Biochem Physiol（1959）37:911#8211;7

3. 毕业设计（论文）进程安排

2017.12-2018.02：文献查阅，完成开题报告 2018.03-2018.04：熟悉微生物操作方法，熟悉实验室仪器的使用，开展实验 2018.04-2018.05：测定发酵产物 2018.05-2018.06：撰写毕业论文