1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier、ncbi电子期刊，springer link全文电子期刊，pubmed等检索资源上查阅关于紫色杆菌素、janthinobacterium菌株、质粒构建等方面的相关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] Choi SY, Yoon K, Lee JI Mitchell RJ Violacein: properties and production of a versatile bacterial pigment. BioMed Res Int.2015，2015: 1-8. [2] Zhang X, Enomoto K. Characterization of a gene cluster and its putative promoter region for violacein biosynthesis in Pseudoalteromonas sp. 520P1[J]. Applied microbiology and biotechnology.2011，90(6): 1963-1971. [3] Wang H, Wang F, Zhu X, et al. Biosynthesis and characterization of violacein, deoxyviolacein and oxyviolacein in heterologous host, and their antimicrobial activities[J]. Biochemical Engineering Journal. 2012，67: 148-155. [4] Prabhu N R M , Min X , Wen-Jun L . Fungal and Bacterial Pigments: Secondary Metabolites with Wide Applications[J]. Frontiers in Microbiology, 2017, 8:1113. [5] Lu Y , Wang L , Xue Y , et al. Production of violet pigment by a newly isolated psychrotrophic bacterium from a glacier in Xinjiang, China[J]. Biochemical Engineering Journal, 2009, 43(2):135-141. [6] Kumar R , Acharya V , Singh D , et al. Strategies for high-altitude adaptation revealed from high-quality draft genome of non-violacein producing Janthinobacterium lividum ERGS5:01[J]. Standards in Genomic Sciences, 2018, 13(1):11. [7] Zhidong Z , Min C , Qiyong T , et al. Identification of a Blue Pigment Producing Strain and Characterization of Produced Pigment[J]. Food Science, 2012, 33(19):236-239. [8] Avgu#353;tin A J, Bertok D Z, Avgu#353;tin G, et al. Isolation and characterization of a novel violacein-like pigment producing psychrotrophic bacterial speciesJanthinobacterium svalbardensissp. nov[J]. Antonie van Leeuwenhoek, 2013, 103(4):763-769. [9] Ahmad W A , Yusof N Z , Nordin N , et al. Production and Characterization of Violacein by Locally IsolatedChromobacterium violaceumGrown in Agricultural Wastes[J]. Applied Biochemistry and Biotechnology, 2012, 167(5):1220-1234. [10] Wang H , Wang F , Zhu X , et al. Biosynthesis and characterization of violacein, deoxyviolacein and oxyviolacein in heterologous host, and their antimicrobial activities[J]. Biochemical Engineering Journal, 2012, 67: 148-155. [11] Fang M Y , Zhang C , Yang S , et al. High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway[J]. Microbial Cell Factories, 2015, 14(1):8. [12] Yikang Z , Ming-Yue F , Gang L , et al. Enhanced Production of Crude Violacein from Glucose in Escherichia coli by Overexpression of Rate-Limiting Key Enzyme(S) Involved in Violacein Biosynthesis[J]. Applied Biochemistry and Biotechnology, 2018. [13] Jiang P X , Wang H S , Zhang C , et al. Reconstruction of the violacein biosynthetic pathway from Duganella sp. B2 in different heterologous hosts[J]. Applied Microbiology and Biotechnology, 2010, 86(4):1077-1088. [14] Yang C , Jiang P , Xiao S , et al. Fed-batch fermentation of recombinant Citrobacter freundii with expression of a violacein-synthesizing gene cluster for efficient violacein production from glycerol[J]. Biochemical Engineering Journal, 2011, 57(none):55-62. [15] Fang M , Wang T , Zhang C , et al. Intermediate-sensor assisted push-pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli[J]. Metabolic Engineering, 2016, 33:41-51.

3. 毕业设计（论文）进程安排

2018.12.20-2019.1.9 文献查询和资料阅读 2019.1.9-2019.1.16 研究方案制定和开题报告写作 2019.1.16-2019.2.25 实践内容的准备 2019.2.25-2019.5.10 实践 2019.5.10-2019.5.15 实践内容处理与结果分析 2019.5.16-2019.6.1 毕业论文书写