1. 毕业设计（论文）的内容和要求

1.明确实验任务，拟定实验方案根据所查阅文献的内容，确定实验内容和方案，拟定科学可行的研究计划； 2.开展实验研究掌握厌氧菌培养的基本操作，设计实验方案，并按计划进行实验； 3.处理实验数据及样品检测实验结束后，进行科学的记录和数据处理，对实验结果进行分析，归纳总结，提出改进方案和可行建议，得出相应的结论； 4.撰写毕业论文对实验过程和数据进行归纳整理，系统分析并得出一般性结论，按照国家标准撰写毕业论文。

2. 参考文献

[1] Feng TC, Cui CZ, Dong F, Feng YY, Liu YD, Yang XM. Phenanthrene biodegradation by halophilic Martelella sp. AD-3 [J]. Journal of applied microbiology. 2012;113(4):779-89. [2] Himmelberg AM, Bruls T, Farmani Z, Weyrauch P, Barthel G, Schrader W, et al. Anaerobic degradation of phenanthrene by a sulfate-reducing enrichment culture [J]. Environmental microbiology. 2018;20(10):3589-600. [3] Meckenstock RU, Boll M, Mouttaki H, Koelschbach JS, Cunha Tarouco P, Weyrauch P, et al. Anaerobic Degradation of Benzene and Polycyclic Aromatic Hydrocarbons [J]. Journal of molecular microbiology and biotechnology. 2016;26(1-3):92-118. [4] Tsai JC, Kumar M, Chang SM, Lin JG. Determination of optimal phenanthrene, sulfate and biomass concentrations for anaerobic biodegradation of phenanthrene by sulfate-reducing bacteria and elucidation of metabolic pathway [J]. Journal of hazardous materials. 2009;171(1-3):1112-9. [5] Tsai JC, Kumar M, Lin JG. Anaerobic biotransformation of fluorene and phenanthrene by sulfate-reducing bacteria and identification of biotransformation pathway [J]. Journal of hazardous materials. 2009;164(2-3):847-55. [6] Zhao Z, Zhang Y, Wang L, Quan X. Potential for direct interspecies electron transfer in an electric-anaerobic system to increase methane production from sludge digestion [J]. Scientific reports. 2015;5:11094. [7] Gao S, Seo JS, Wang J, Keum YS, Li J, Li QX. Multiple degradation pathways of phenanthrene by Stenotrophomonas maltophilia C6 [J]. International biodeterioration amp; biodegradation. 2013;79:98-104. [8] Keum YS, Seo JS, Hu Y, Li QX. Degradation pathways of phenanthrene by Sinorhizobium sp. C4 [J]. Applied microbiology and biotechnology. 2006;71(6):935-41. [9] Nzila A. Biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons under anaerobic conditions: Overview of studies, proposed pathways and future perspectives [J]. Environmental pollution. 2018;239:788-802. [10] Seo JS, Keum YS, Li QX. Mycobacterium aromativorans JS19b1(T) Degrades Phenanthrene through C-1,2, C-3,4 and C-9,10 Dioxygenation Pathways. International biodeterioration amp; biodegradation. 2012;70:96-103. [11] Shi Z, Zhang F, Wang C. Adsorption of phenanthrene by earthworms - A pathway for understanding the fate of hydrophobic organic contaminants in soil-earthworm systems [J]. J Environ Manage. 2018;212:115-20. [12] Wang C, Huang Y, Zhang Z, Wang H. Salinity effect on the metabolic pathway and microbial function in phenanthrene degradation by a halophilic consortium [J]. AMB Express. 2018;8(1):67. [13] Deng L, Ren Y, Wei C. Pyrene degradation by Pseudomonas sp. and Burkholderia sp. enriched from coking wastewater sludge. Journal of environmental science and health Part A [J], Toxic/hazardous substances amp; environmental engineering. 2012;47(13):1984-91. [14] 孙明明, 滕应, 骆永明. 厌氧微生物降解多环芳烃研究进展[J]. 微生物学报, 2012, 52(8):931-939. [15] 曹军伟. 深海多环芳烃降解菌Celeribacter indicus P73~T降解荧蒽和菲的机制研究[D]. 2016. [16] 李作扬. 石油降解细菌的分离、筛选、鉴定及其对多环芳烃降解性能研究[D]. 2016.

3. 毕业设计（论文）进程安排

2018.12-2019.1 查阅相关文献，了解菲降解菌及生物电化学的研究进展，撰写毕业论文综述部分； 2019.1-2019.3 对菲降解菌Clostridium sp. strain LZ25进行富集培养；进行电化学对Clostridium sp. strain LZ25降解菲的影响实验； 2019.3-2019.4 完成对后期样品菲含量，中间代谢产物，功能酶及功能基因片段等指标的测定及撰写毕业论文。