1. 毕业设计（论文）的内容和要求

（1）掌握文献查阅的一般方法，学会在中国期刊网、web of science科学引文索引、springer link电子期刊、elsevier sdos电子期刊等检索资源上查阅关于生物催化产异槲皮素研究的相关文献，并对大肠杆菌表达系统有全面了解。

（2）文献阅读及综述：阅读与课题相关的中英文文献，了解国内外的研究动态，撰写文献综述。

（3）明确实验任务，拟定实验方案：根据所查阅文献的内容，确定研究内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] Son, Mi Hye , Bong-Gyu Kim , Dae Hwan Kim . Production of Flavonoid O-Glucoside Using Sucrose Synthase and Flavonoid O-Glucosyltransferase Fusion Protein. J. Microbiol. Biotechnol. (2009), 19(7), 709#8211;712. [2] Yi Li, Sandie Baldauf, Eng-Kiat Lim, and Dianna J. Bowles. Phylogenetic Analysis of the UDP-glycosyltransferase Multigene Family of Arabidopsis thaliana. THE JOURNAL OF BIOLOGICAL CHEMISTRY. (2001), Vol. 276, No. 6, 4338#8211;4343. [3] Joe Ross, Yi Li, Eng-Kiat Lim and Dianna J Bowles. Higher plant glycosyltransferases. Genome Biology. 2001, 2(2): reviews3004.1#8211;3004.6. [4] Catherine M. Kramera , Rogerio T.N. Prataa , Michael G. Willitsa , Vincenzo De Lucab , John C. Ste#64256;ensa , Gerson Grasera, *. Cloning and regiospecificity studies of two flavonoid glucosyltransferases from Allium cepa. Phytochemistry. 64 (2003) 1069#8211;1076. [5] Masao Hirotani, Ryo Kuroda, Hideyuki Suzuki, Takafumi Yoshikawa. Cloning and expression of UDP-glucose: avonoid 7-O-glucosyltransferase from hairy root cultures of Scutellaria baicalensis. Planta (2000) 210: 1006-1013. [6] Frederik De Bruyn, Jo Maertens, Joeri Beauprez, Wim Soetaert, Marjan De Mey. Biotechnological advances in UDP-sugar based glycosylation of small molecules. Biotechnology Advances. 33 (2015) 288#8211;302. [7] Lavoie EG, Kukulski F, Levesque SA, Lecka J, Sevigny J. 2004. Cloning and characterization of mouse nucleoside triphosphate diphosphohydrolase-3. Biochem Pharmacol. 67:1917#8211;1926. [8] Eng-Kiat Lim, David A. Ashford, Bingkai Hou, Rosamond G. Jackson, Dianna J. Bowles. Arabidopsis Glycosyltransferases as Biocatalysts in Fermentation for Regioselective Synthesis of Diverse Quercetin Glucosides. Garfield Weston Foundation for CNAP. 2004. [9] Pesnot T, Jorgensen R, Palcic MM, Wagner GK. 2010. Structural and mechanistic basis for a new mode of glycosyltransferase inhibition. Nat Chem Biol. 6:321#8211;323. [10] Teng Y, Liu Q, Ma J, Liu F, Han Z, Wang Y, Wang W. 2006. Cloning, expression and characterization of a novel human CAP10-like gene hCLP46 from CD34( ) stem/progenitor cells. Gene. 371:7#8211;15.

3. 毕业设计（论文）进程安排

2016.1-2016.3 熟悉实验原理和实验操作，查阅文献，对课题进行初步探索。

2016.3-2016.4 采用分子克隆技术，构建糖基转移酶ugt73g1与蔗糖合酶sus共表达重组质粒。

2016.4-2016.5 对优良重组菌使用乳糖自诱导培养基进行诱导表达，验证酶功能，并建立高效生物催化产异槲皮素体系。